Lea Hürlimann

Dr. Lea Huber-Hürlimann

Postdoctoral Research Assistant

Tel.: +41 44 634 27 99



Curriculum vitae

Education and working experience
2013-2017 Ph.D. Student, Institute of Medical Microbiology, University of Zurich (Prof. M. A. Seeger)
2011-2013 Master in Science, Biochemistry, University of Zurich
2007-2011 Bachelor in Science, Biochemistry, University of Zurich
2003-2007 Kantonales Kurzzeitgymnasium Menzingen, Zug, Matura

Research interests

  • Functional characterization of ABC exporters using in vitro assays, in vivo transport and drug resistance in knockout strains
  • Structure determination of membrane proteins using in vitro selected binders


The ABC exporter IrtAB imports and reduces mycobacterial siderophores.
Arnold FM, Weber MS, Gonda I, Gallenito MJ, Adenau S, Egloff P, Zimmermann I, Hutter CAJ, Hürlimann LM, Peters EE, Piel J, Meloni G, Medalia O, Seeger MA (2020)
Nature 580: 413-417

The extracellular gate shapes the energy profile of an ABC exporter.
Hutter CAJ, Timachi MH, Hürlimann LM, Zimmermann I, Egloff P, Göddeke H, Kucher S, Štefanić S, Karttunen M, Schäfer LV, Bordignon E, Seeger MA (2019)
Nat Commun 10: 2260

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG.
Hohl M, Remm S, Eskandarian HA, Dal Molin M, Arnold FM, Hürlimann LM, Krügel A, Fantner GE, Sander P, Seeger MA (2019)
Mol Microbiol 111:1263-1282

Split tasks of asymmetric nucleotide-binding sites in the heterodimeric ABC exporter EfrCD.
Hürlimann LM, Hohl M, Seeger MA (2017)
FEBS J 284: 1672-1698

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis.
Hürlimann LM, Corradi V, Hohl M, Bloemberg GV, Tieleman DP, Seeger MA (2016)
Antimicrob Agents Chemother 60: 5400-5411

A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.
Bukowska MA, Hohl M, Geertsma ER, Hürlimann LM, Grütter MG, and Seeger MA (2015)
Biochemistry 54: 3086–3099

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter.
Hohl M, Hürlimann LM, Böhm S, Schöppe J, Grütter MG, Bordignon E, Seeger MA (2014)
Proc Natl Acad Sci USA 111: 11025-30.