UZH - Institut für Medizinische Mikrobiologie - Prof. Dr. Peter Sander, Ph.D.

Prof. Dr. Peter Sander, Ph.D.
Research Group Leader
Tel.: +41 44 634 26 84
psander@imm.uzh.ch
Website

Curriculum vitae

Name

Peter Sander,
Prof. Dr. rer. nat.
Phone +41 44 634 26 84
psander@imm.uzh.ch

University Degree

Diploma in biology, University of Munster, Germany PhD, University of Munster Habilitation, Hannover Medical School, Germany and University of Zurich, Switzerland

Education

1983 – 1990	Studies of biology, University of Munster
1993	Dr. rer. nat. (PhD), University of Munster
2001	Habilitation, Hannover Medical School; venia legendi: Microbiology
2004	Habilitation, University of Zurich; venia legendi: Microbiology

Professional experience

1990 – 1993	PhD student, Institute for General Zoology and Genetics, University of Munster
1993 – 1998	Postdoc, Department of Medical Microbiology, Hannover Medical School
1998 – 2001	Research Assistant, Department of Medical Microbiology, Hannover Medical School
2001 – today	Principle Investigator and Group leader, Institute of Medical Microbiology, University of Zurich

Research area

Mycobacterium tuberculosis: Genetics, virulence, host-pathogen interaction, protein secretion, lipoprotein synthesis and transport, genetics
Vaccine development
Antibiotics and tuberculosis drugs: drug development, activity, resistance, specificity, toxicity

Awards/Honours

Young investigator award of the German Society for Hygiene and Microbiology (DGHM) 2002

List of publications

Original articles (peer reviewed)

Mycobacteria virulence and genetics

Rampini, S.K., Selchow, P., Keller, C., Ehlers, S., Böttger, E.C., and Sander P. (2008) LspA-inactivation in Mycobacterium tuberculosis results in attenuation without affecting phagosome maturation arrest. Microbiology (in press).

Master, S.S., Rampini, S.K., Davis, A.S., Keller, C., Ehlers, S., Springer, B., Timmins, G.S., Sander, P.*, and Deretic, V*. (2008) Mycobacterium tuberculosis prevents inflammasome activation. Cell Host Microbe 3: 224 232. (*co-corresponding authors)

Güthlein, C., Wanner, R.M., Sander, P., Böttger, E.C., and Springer, B. (2008) A mycobacterial smc null mutant is proficient in DNA repair and long-term survival. J. Bacteriol. 190: 452 456.

Rezwan, M., Laneelle, M.A., Sander, P.*, and Daffe, M. (2007) Breaking down the wall: fractionation of mycobacteria. J. Microbiol. Meth. 68: 32 39.

(* corresponding author)

Dittrich, D., Keller, C., Ehlers, S., Schultz, J.E., and Sander, P. (2006) Characterization of a Mycobacterium tuberculosis mutant deficient in pH-sensing adenylate cyclase Rv1264. Intern. J. Med. Microbiol. 296: 563 566.

Guo, Y.L., Kurz, U., Schultz, A., Linder, J.U., Dittrich, D., Keller, C., Ehlers, S., Sander, P., and Schultz, J.E. (2005) Interaction of Rv1625c, a mycobacterial class IIIa adenylyl cyclase with a mammalian congener. Mol. Microbiol. 57: 667 677.

Springer, B., Sander, P., Sedlacek, L.,Hardt, W.-D., Mizrahi, V., Schaer, P., and Böttger, E.C. (2004) Lack of mismatch correction designs genome evolution in mycobacteria. Mol. Microbiol. 53: 1601 1609.

Sander, P., Rezwan, M., Walker, B., Rampini, S.K., Kroppenstedt, R., Ehlers, S., Keller, C., Keeble, J.R., Hagemeier, M., Colston M.J., Springer, B., and Böttger, E.C. (2004) Lipoprotein processing is required for virulence of Mycobacterium tuberculosis. Mol. Microbiol. 52: 1543 - 1552.

Rand, L., Hinds, J., Springer, B., Sander, P., Buxton, R.S., and Davis, E.O. (2003) The majority of inducible DNA repair genes in Mycobacterium tuberculosis are induced independently of RecA. Mol. Microbiol. 50: 1031 1042.

Davis, E.O., Springer, B., Gopaul, K.K., Papavinasasundaram, K.G., Sander, P., and Böttger, E.C. (2002) DNA damage induction of recA in Mycobacterium tuberculosis independent of RecA and LexA. Mol. Microbiol. 46: 791 - 800.

Master, S.S., Springer, B., Sander, P., Böttger, E.C., Deretic, V., and Timmins, G.S. (2002) Oxidative stress response genes in Mycobacterium tuberculosis: Role of ahpC in resistance to peroxynitrite and stage-specific survival in macrophages. Microbiol. 148: 3139 3144.

Raynaud, C., Papavinasasundaram, K.G., Speight, R.A., Springer, B., Sander, P., Böttger, E.C., Colston, M.J., and Draper, P. (2002) The functions of OmpATb, a pore-forming protein of Mycobacterium tuberculosis. Mol. Microbiol. 46: 191 - 201.

Springer, B., Master, S., Sander, P., Zahrt, T., McFalone, M., Song, J., Papavinasasundaram, K.G., Colston, M.J., Böttger, E.C., and Deretic, V. (2001) Silencing of oxidative stress response in Mycobacterium tuberculosis: Expression patterns of ahpC in virulent and avirulent strains and effect of ahpC inactivation. Infect. Immun. 69: 5967 5973.

Frischkorn, K., Springer, B., Böttger, E.C., Davis, E.O., Colston, M.J., and Sander, P. (2000) In vivo splicing and functional characterization of Mycobacterium leprae RecA. J. Bacteriol. 182: 3590 - 3592.

Sander, P., Alcaide, F., Richter, I., Frischkorn, K., Tortoli, E., Springer, B., Telenti, A., and Böttger, E.C. (1998) Inteins in mycobacterial GyrA are a taxonomic character. Microbiol. 144: 589 591.

Frischkorn, K., Sander, P., Scholz, M., Teschner, K., Prammananan, T., and Böttger, E.C. (1998) Investigation of mycobacterial recA function: protein introns in the RecA of pathogenic mycobacteria do not affect competency for homologous recombination. Mol. Microbiol. 29: 1203 - 1214.

Sander, P., Meier, A., and Böttger, E.C. (1995) rpsL⁺ : a dominant negative selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16: 991 - 1000.

Vaccine development

Keller, P., Böttger, E.C., Sander, P. (2008) Tuberculosis vaccine strain Mycobacterium bovis BCG Russia is a natural recA mutant. BMC Microbiology 8: 120.

Sander, P., Böttger, E.C., Springer, B., Steinmann, B., Rezwan, M., Stavropoulos, E., and Colston, M.J. (2003) A recA deletion mutant of Mycobacterium bovis BCG confers protection equivalent to that of wild-type BCG but shows increased genetic stability. Vaccine 21: 4124 4127.

Springer, B., Sander, P., Sedlacek, L., Ellrott, K., and Böttger, E.C. (2001) Instability and site-specific excision of integration-proficient mycobacteriophage L5 plasmids: development of stably maintained integrative vectors. Int. J. Med. Microbiol. 290: 669 675.

Sander, P., Papavinasasundaram, K.G., Dick, T., Stavropoulos, E., Ellrott, K., Springer, B., Colston, M.J., and Böttger, E.C. (2001) Mycobacterium bovis BCG recA deletion mutant shows increased susceptibility to DNA-damaging agents but wild-type survival in a mouse infection model. Infect. Immun. 69: 3562 - 3568.

Antibiotics

Weber, W., Schoenmakers, R., Keller, B., Grau, T., Gitzinger, M., Grau, T., Daoud-El Baba, M., Sander, P., and Fussenegger, M. (2008). A synthetic mammalian gene circuit reveals anti-tuberculosis compounds. Proc. National Acad. Sci. USA 105: 9994 9998.

Hobbie, S.N., Kalapala, S.K., Subramanian, A., Bruell, C., Schmidt, S., Dabow, S., Vasella, A., Sander, P., and Böttger, E.C. (2007) Engineering the rRNA decoding site of eukaryotic cytosolic ribosomes in bacteria. Nucl. Acids Res. 35: 6086 6093.

Hobbie, S.N., Pfister, P., Bruell, C., Sander, P., Francois, B., Westhof, E., and Böttger, E.C. (2006) Binding of neomycin-class aminoglycoside antibiotics to mutant ribosomes with alterations in the A site of 16S rRNA. Antimicrob. Agents Chemother. 50: 1489 1496.

Sander, P., Belova, L., Kidan, Y.G., Pfister, P., Mankin A.S, and Böttger, E.C. (2002) Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations. Mol. Microbiol. 46: 1295 - 1304.

Sander, P., Springer, B., Prammananan, T., Sturmfels, A., Kappler, M., Pletschette, M., and Böttger, E.C. (2002) Fitness cost of chromosomal drug resistance conferring mutations. Antimicrob. Agents Chemother. 46: 1204 - 1211.

Böttger, E.C., Springer, B., Prammananan, T., Kidan, Y., and Sander, P. (2001) Structural basis for selectivity and toxicity of ribosomal antibiotics. EMBO Rep. 2: 318 - 323.

Springer, B., Kidan, Y.G., Prammananan, T., Ellrott, K., Böttger, E.C. and Sander, P. (2001) Mechanisms of streptomycin resistance: Selection of mutations in the 16S rRNA gene conferring resistance. Antimicrob. Agents Chemother. 45: 2877 2884.

Xiong, L., Polacek, N., Sander, P., Böttger, E.C., and Mankin, A. (2001) pKa of adenine 2451 in the ribosomal peptidyl transferase center remains elusive. RNA 7: 1365 - 1369.

Sander, P., De Rossi, E., Böddinghaus, B., Cantoni, R., Branzoni, M., Böttger, E.C., Takiff, H., Rodriquez, R., Lopez, G., and Riccardi, G. (2000) Contribution of the multidrug efflux pump LfrA to innate mycobacterial drug resistance. FEMS Microbiol. Lett. 193: 19 - 23.

Prammananan, T., Sander, P., Springer, B., and Böttger, E.C. (1999) RecA-mediated gene conversion and aminoglycoside resistance in strains heterozygous for rRNA. Antimicrob. Agents Chemother. 43: 447 - 453.

Böttger, E.C., Springer, B., Pletschette, M., and Sander, P. (1998) Fitness of antibiotic-resistant microorganisms and compensatory mutations. Nature Med. 4: 1343 - 1344.

Prammananan, T., Sander, P., Brown, B.A., Frischkorn, K., Onyi, G.O., Zhang, Y., Böttger, E.C., and Wallace, R.J., Jr. (1998) A single 16S ribosomal RNA substitution is responsible for resistance to amikacin and other 2-deoxystreptamine aminoglycosides in Mycobacterium abscessus and Mycobacterium chelonae. J. Infect. Dis. 177: 1573 - 1581.

Sander, P., Prammananan, T., Meier, A., Frischkorn, K., and Böttger, E.C. (1997) The role of ribosomal RNAs in macrolide resistance. Mol. Microbiol. 26: 469 - 480.

Sander, P., Prammananan, T., and Böttger, E.C. (1996) Introducing mutations into a chromosomal rRNA gene using a genetically modified eubacterialhost with a single rRNA operon. Mol. Microbiol. 22: 841 - 848.

Meier, A., Sander, P., Schaper, K.J., Scholz, M., and Böttger, E.C. (1996) Correlation of molecular resistance mechanisms and phenotypic resistance levels in streptomycin-resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 40: 2452 - 2454.

Meier, A., Heifets, L., Wallace, R.J., Jr., Zhang, Y., Brown, B.A., Sander, P., and Böttger, E.C. (1996) Molecular mechanisms of clarithromycin resistance in Mycobacterium avium: observations of multiple 23S rDNA mutations in a clonal population. J. Infect. Dis. 174: 354 - 360.

Wallace, R.J., Jr., Meier, A., Brown, B.A., Zhang, Y., Sander, P., Onyi, G.O., and Böttger, E.C. (1996) Genetic basis for clarithromycin resistance among isolates of Mycobacterium chelonae and Mycobacterium abscessus. Antimicrob.Agents Chemother. 40: 1676 - 1681.

Review articles

Sander, P., Langert, W., and Müller, K. (1993) Mechanisms of upstream activation of the rrnD promoter P₁ of Escherichia coli. J. Biol. Chem. 268: 16907 16916.

Rezwan, M., Grau, T., Tschumi, A., and Sander, P. (2007) Lipoprotein synthesis in mycobacteria. Microbiology 153: 652 658.

Sander, P., and Böttger, E.C. (1999) Mycobacteria: Genetics of resistance and implications for treatment. Chemotherapy 45: 95-108.

Sander, P., Meier, A., and Böttger, E.C. (1996) Mechanisms of drug resistance in mycobacteria. Res . Microbiol. 147: 59-67.

Book chapters

Reyrat, J.M., Sander, P., Bardarov. S., and Stoker, N. (2005) Gene replacement systems in Mycobacterium tuberculosis. In: Tuberculosis and the tubercle bacillus. Eds. Cole, S.T., Eisenach, K.D., McMurray, D.N. and Jacobs, W.R., ASM Press, Washington, DC, p. 183 190.

Sander, P., and Böttger, E.C. (2001) Gene replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG. In: Methods in Molecular Biology “Mycobacteria Protocols”. Eds. Parish, T., and Stoker, N.G., Humana Press, p. 93-104.

Sander, P., and Böttger, E.C. (1998) Gene replacement in Mycobacterium smegmatis using a dominant negative selectable marker. Methods Mol. Biol. 101: 207-216.